Bacterial transformation video in hindi 5K Views. Mar 19, 2022 · #transformation #Prof_masood_fuzail #Griffith_Experiments #Transformation_Principle In this video, I have defined and explained the process of transformation in bacteria in a very simple and easy way. Nutrient broth incubation allows beta lactamase expression GFP Cell wall Beta lactamase (ampicillin resistance) Natural bacterial transformation involves the internalization and chromosomal integration of DNA and has now been documented in ~80 species. Teachers ca Find Bacterial Transformation stock video, 4k footage, and other HD footage from iStock. Bacterial transformation is a process where bacteria absorb DNA from their environment resulting in new characteristics in the bacteria. It is one of the cornerstone of molecu Apr 7, 2021 · 50. Here, only the restricted genome has the possibility of entering the recipient cells. In essence, what happened in these circumstances is bacterial transformation. The genetic recombination Genetics, Microbial (general)☆ John E. Genetic recombination occurs between homologous chromosomes. The transfer of genes from one cell to another by a bacteriophage is known as __________________ a) Recombination b) Conjugation c) Transduction d) Transformation 2. Transformation, transduction, and conjugation occur in nature as forms of HGT, but transfection is unique to the lab. Step by Step Tutorial for our pGreen Bacterial transformation Biolab (SKU: BT1. Apr 27, 2019 · 3. In order to develop an effective vaccine against pneumonia, Frederick Griffith set out to study two different strains of the Streptococcus pneumoniae: a non-virulent strain with a rough appearance (R-strain) and a virulent strain with a smooth appearance (S-strain) due to Jul 19, 2019 · The introduction of DNA into bacteria by transformation is an essential step in the construction of recombinant strains. • energy-requiring developmental process. Though b Sep 2, 2019 · Introducing Southern Biological Tutorial series. For transformation to occur, the recipient bacteria must be in a state known as competence. Bacterial transformation meaning in Hindi (हिन्दी मे मीनिंग ) is जीवाणु रूपांतरण. edu/resources/dnalc-live/This experiment illustr "In this video, we will explore the fascinating world of bacterial genetic exchange through the mechanisms of transduction, conjugation, and transformation. Processes for bacterial transformation based on the Yoshida Effect can utilize sepiolite as an acicular nanofiber. The objective is to obtain the replication of sequence of interest of a recombinant Nov 15, 2024 · used to manufacture insulin, which can be used for insulin therapy by diabetics. 9. facebook. Bacterial transformation is used in gene mapping by determining the proximity of genes on a DNA molecule. coli bacteria, students use the results to draw a scientific model that explains how the fluorescent bacteria were produced. • Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. The process involves four main stages: competence development, DNA binding, DNA uptake and processing, and integration into the host genome. Dec 24, 2022 · जाने transduction in bacteria in hindi , पारक्रमण क्या है जीवाणु में रूपान्तरण , transformation in bacteria in hindi ? रूपान्तरण (Transformation) Watch Transformation in Hindi from Transduction, Transformation and Conjugation here. May 6, 2020 · Bacterial Transformation is a mechanism of uptake of bacterial DNA from one cell of Bacteria to another. In its natural state, the competency of E. E. Wertz, K. 4 Natural Transformation The process by which bacteria are able to take up and integrate exogenous free DNA from the environment is termed as natural transformat in bacteria [7]. #transformatio This video series is about the bacterial genetics involving both the horizontal gene transfer and the vertical gene transfer. Transformation: Splicing gene(s) from one organism and inserting them into another. “Horizontal Transfer of Antibiotic Resistance Genes in Clinical Environments. southernbiological. Jan 29, 2019 · 62. Aug 31, 2019 · One thought on “ बैक्टीरिया में प्रजनन #Reproduction in Bacteria in Hindi ” Mendy Velotta. coli has made possible the cloning of many genes, including human genes, and the development of the biotechnology industry. org/ to download this lesson and more!This lesson is a remote learning version of our hands-on lab. Jan 29, 2019 · Bacterial Transformation using Plasmids. Submit Feedback About Us. Sc 1st year Zoology New Syllabus all videos link 👇👇👇👇👇👇👇👇https://youtube. The significance of natural transformation [8] lies in the ecological, evolution Jan 31, 2024 · Transformation is the process of introduction of derived DNA fragments from a donor bacteria into a recipient bacteria. Facebook: https://www. Watch our scientific video articles. biotechnologylessons. org/ to download this lesson and more! Watch Reproduction in Bacteria by Binary Fission in Hindi from Bacteria and Archaea and Reproduction and Variation and Fission and Transduction, Transformation and Conjugation and Microorganisms - Bacteria and Reproduction and Sexuality in Organisms and Asexual Modes of Reproduction here. The result is the R strain acted in the same way as the S strain, became pathogenic, and caused death in the mice. In the online version, students watch point of view videos to identify the necessary steps for a successful bacterial transformation. Background In early 20th century, pneumonia was accountable for a large portion of infectious disease deaths1. Electroporation has applications in biotech for bacterial, yeast, and plant transformation, as well as gene therapy, cell therapy, and tumor treatment. जीवाणु (Bacteria) सूक्ष्म जीव हैं जो प्रायः एककोशिकीय होते हैं। ये In this lecture, we will discuss transformation in bacteria, the discovery of transformation, and the mechanism of transformation. Competence for transformation is typically induced by: • high cell density • nutritional limitation, • conditions associated with the stationary phase of bacterial growth. प्रौद्योगिकी और इसकी प्रक्रिया | Read this article in Nov 14, 2022 · टाइफायड – Typhoid Bacterial diseases in Hindi टाइफाइड, साल्मोनेला टाइफी (Salmonella typhi) नामक बैक्टीरिया के कारण होने वाला रोग है। इस बीमारी को मोतीझरा, आंत्र ज्वर (enteric fever) या मियादी Aug 1, 2019 · 50. Prior to transformation, recombinant plasmids are constructed by inserting the DNA sequence of interest into a vector as described in traditional cloning basics . The ability to deliberately transform the bacterium E. Research. Sc 1st year Feb 13, 2022 · The video gives a description of genetic recombination in bacteria over viewing Transformation, transduction and conjugation Significance of 'heat shock' method in bacterial transformation is to facilitate: (a) Binding of DNA to the cell wall (b) Uptake of DNA through membrane transport proteins (c) Uptake of DNA through transient pores in the bacterial cell wall (d) Expression of antibiotic resistance gene In this video Bacterial transformation is explained in detail, the content is for CSIR NET exam. Dec 16, 2021 · #griffithobservatory #biology After transforming E. Students then analyze pictures of actual experimental results to Jan 11, 2021 · In this video, the concepts of bacterial transformation, conjugation and transduction are discussed in an animated way for easy understanding. Helpful video in the preparation of GATE and CSIR Genetic recombination in Bacteria, transformation, Transduction, conjugation, lecture in URDU/HINDI# Genetic recombination in bacteria # transformation, Tr Watch this Scientific Journal Video about Bacterial Transformation: Electroporation (Translated to Hindi) at JoVE. Scientists then expose the bacteria to antibiotics. Scheme of steps involved in studying natural transformation of bacteria 2. Transformation. com/ What Is Transformation In Bacteria? In this informative video, we’ll dive into the fascinating world of bacterial transformation. com In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic Due to this, the phage genome sometimes carries the bacterial genome with it and integrates it into the genome of the recipient cell. Surviving bacterial colonies should contain the plasmid because the plasmid contains an antibiotic resistance gene. 2. -What is Genetic Recombination in Bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. The transformation process has four general steps: 1) Preparation of competent cells, 2) Incubation with DNA, 3) Recovery of the cells and 4) Plating cells for growth of the transformants. Scientists fragment the DNA and incubate it with competent bacteria. Oct 15, 2012 · Table of Contents:00:26 - Central Framework/Dogma of Molecular Biology00:58 - Bacterial Transformation02:59 - 04:11 - 06:22 - Transformation is a key process in molecular cloning, by which multiple copies of recombinant DNA molecules are produced. 00)www. This lacture is delivered in Hindi by Himanshi. 10. a) True b) False 3. रीकॉम्बिनेण्ट डी. Recent advances have established that phylogenetically distant species share conserved uptake and processing proteins but differ in the inducing cues and regul … Apr 30, 2023 · 113. Bacterial Transformation Genetic Engineering: Any manipulation of genetic material within a cell or organism. Researchers first insert a DNA fragment, such as a gene, onto a plasmid, a circular piece of DNA, in a conventional cloning procedure. s. Watch Conjugation in Hindi from Transduction, Transformation and Conjugation and Microorganisms - Bacteria and Bacteria and Archaea here. Bacterial transformation is the transfer of free DNA released from a donor bacterium into the extracellular environment that results in assimilation and usually an expression of the newly acquired trait in a recipient bacterium. Click for more examples 1. This process occurs in four main steps: (1) Binding of double-stranded DNA to cell surface receptors, (2) Entry of a single strand of DNA into the cell while the complementary strand is degraded, (3) Integration of the single-stranded DNA into the bacterial In this video we will discuss about Transformation vs Transduction vs Conjugation in Bacteria | Horizontal Gene Transfer or Lateral Gene Transfer in Bacteria This video is part of BABEC's "Bacterial Transformation" lab. Link to purchase these NOTES :http://www. in the transformation process bacteria w Apr 12, 2021 · Hey Friends,Transformation, Conjugation and Transduction are ways of bacteria to transfer genetic material horizontally. Swirl bacteria in each tube containing transforming solution to distribute bacteria throughout the solution. Great video footage that you won't find anywhere else. me/AnuragsainiMBBSS#transformationinbacteria #microbiology #mbbslectures Transformation in bacteria Hello friends welcome back to our YouTube channel KBS Biology. genetics,bacterial genetics,bacteria,bacterial,bacterial genetics in hindi,genetic,fundamental bacterial genetics,bacterial genetics microbiology,transpositi JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. This process was first observed in Streptococcus pneumonia by Griffith. explained in Hindi. g. Sep 13, 2024 · Bacterial Diseases Hindi, जीवाणु जनित रोगों जैसे कॉलरा, निमोनिया, टाइफाइड, टिटनेस आदि के बारे में जाने जो NEET एग्जाम के लिए उपयोगी है Mar 25, 2018 · In this animation produced by WGBH and Digizyme, Inc. This process is referred to as bacterial transformation because of the replication of DNA and protein synthesis. , learn about bacterial transformation, the process by which DNA plasmids are introduced into a bacteria DNALC Live: Online educational programming to engage educators, students, and the public. Jun 1, 2021 · This video is all about three ways of gene transfer in bacteria. Take an inoculating loop and remove a single colony of bacteria from a freshly streaked plate grown overnight. , learn about bacterial transformation, the process by which DNA plasmids are introduced into a bacteria This video provides all the details about the bacterial cell transformation. The term “transformation” refers cellular ingestion of foreign DNA. Behavior; Biochemistry; Bioengineering; Biology; Cancer Research; Chemistry; Developmental Biology Dec 10, 2024 · Unlock the mysteries of #bacterialtransformation! In this video, we'll dive into the fascinating world of bacteria secrets and explore what happens during th Bacterial transformation is a process in which foreign DNA is introduced into bacterial cells, resulting in the acquisition of new genetic traits. • In order for a bacterium to bind, take up and recombine exogenous DNA into its chromosome, it must become competent. Fig. The ability to take up free, extracellular genetic material is the prerequisite for bacterial competent cells to undergo transformation. coli is very low (10-5 −10-10) [1] thus cells must be made competent for efficient transformation. These microorganisms have a unique ability to absorb foreign DNA […] Bacterial Transformation using Plasmids. Watch all CBSE Class 5 to 12 Video Lectures here. #GramPositiveBacteria #Streptococcus infection #culture_and_diagnosis in HindiHi,I Am #PremBhatiawelcome to our you tube channel. ” Canadian Journal of Microbiology 65 (1): 34–44. कोलाई = एसरिकिया कोलाई) में प्रदर्शित की गई । इनमें In this video we will discus about Genetic Recombination and its Mechanism Transformation In detail. org Mar 23, 2021 · For each plasmid, obtain 250μl of transformation buffer (50mM CaCl 2) in microfuge tubes and place on ice for 10 minutes. Bacterial recombination causes In molecular cloning we take a gene from one place and stick it into a vector which serves as a “vehicle” to take that gene into cells. This phenomena has significant ramifications for health care, agriculture, and environmental science and has evolved into a fundamental genetic engineering tool. Transformation in bacteria is a type of Get access to the latest Bacterial Transformation and Competence (in Hindi) prepared with CSIR-UGC NET course curated by Jyoti Kumari on Unacademy to prepare for the toughest competitive exam. Log In. The genes In the following experiment, you will insert a plasmid into E. This process allows bacteri E. The rDNA which is an exogenous DNA, is required to be inserted and expressed in About Press Copyright Contact us Creators Advertise Developers Terms Privacy Press Copyright Contact us Creators Advertise Developers Terms Privacy Sep 23, 2020 · Register for a free account at https://babec. LIKE | COMMENT | SHARE | SUBSCRIBEStay connected with HELP TV to learn and watch more interesting and learning videos. May 19, 2021 · Bacterial Transformation Definition. Transformation is the process of DNA uptake by the bacteria from the surrounding environment. 113. In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Video Reff: www. Nov 7, 2021 · B. EN - English This Lecture describes the Bacterial Transformation in very simple manner. Bacterial Transformation - 1 (in Hindi) 10m 27s. 50. In the 1930s, the French enologist Jean Rib reau-Gayon published papers stating Know answer of question : what is meaning of Bacterial transformation in Hindi? Bacterial transformation ka matalab hindi me kya hai (Bacterial transformation का हिंदी में मतलब ). It also explains the utility of the Heat Shock and also Cold Treatment. Website: This video explains the principle behind the bacterial transformation Learn by watching this video about Bacterial Transformation using Plasmids - Concept at JoVE. Bacterial Transformation and Competence (in Hindi) 10m 02s This is the first part of a series of videos on bacterial genetics. , a plasmid) from their surroundings. Feb 2, 2022 · in this video we will discuss about microbial genetics in Hindi if you like this video plz like share comment and subscribe my YouTube channel 👉👉sourc Jan 23, 2023 · Bacterial recombination is characterized by DNA transfer from one “donor” organism to a “recipient” organism, which allows the bacteria to express new protei Aug 1, 2019 · 50. 6K Views. Brooks Low, in Reference Module in Biomedical Sciences, 2017 Transformation. com Sep 5, 2019 · ⦁ II) लैंगिक पुनर्योजन (Genetic recombination) – जीवाणुओं में लैंगिकता सर्वप्रथम टेटम तथा लीडरबर्ग द्वारा Escherichia coli (E. learner. In nature, this genetic material often comes from About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features NFL Sunday Ticket Press Copyright Transformation in Bacteria Griffith ExperimentTransformation in BacteriaTransformation in Bacteria in HindiTransformation in Bacteria AnimationTransformation Jul 26, 2017 · this video will teach you all about the reproduction in bacteria in hindi. today we will discuss about transformation in bacteria. com in this video we will discuss about bacterial transformation if you like this video plz like share comment and subscribe my YouTube channel 👉sourcebotany ☺b Learn by watching this video about Bacterial Transformation using Plasmids - Procedure at JoVE. Bacterial transformation involves the DNA repair. ए. There are 27 example sentences for bacterial transformation. cshl. On the other hand, bacterial transformation is clearly an adaptation for transfer of DNA between bacteria of the same species. Gold Biotechnology (U. Similar thing is done artificially in lab to transfer gene of interest in living bacteria. Transformation is the uptake of genetic material from the environment by bacterial cells. Learn about the Strain improvement | Mutation | Protoplast fusion | RDT | genetic cloning | Transformation | HindiSubscribe us: https://youtube. Bacteria usually gets a bad reputation; however, it is incredibly useful thanks to its versatility. Bacteria capable of taking up DNA are known as competent cells. Because of this, nearly all plasmids (even those designed for mammalian cell expression) carry both a bacterial origin of replication and an antibiotic resistance gene Understanding Bacterial Transformation. Jan 10, 2017 · The process of bacterial transformation is also a step of pivotal importance in the field of genetic engineering. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. com Learn by watching this video about DNA Cloning and Bacterial Transformation at JoVE. In this video, we learned about the basic principles of bacterial transformation. 1. बैक्टीरियल ट्रांसफॉर्मेशन (Bacterial Transformation) वह प्रक्रिया है जिसके E. Cameron. Aug 13, 2021 · For notes join our telegram channel https://t. Registration No 3,257,926) are registered trademarks of Gold Biotechnology, Inc. The plasmid you will use carries the gene bla, which codes for a protein (beta-lactamase) that is secreted by the bacteria, inactivating the ampicillin in the agar and allowing for bacterial growth. Bacterial transformation is a naturally occurring process, in which bacteria ingest foreign DNA and then amplify or clone it. Many bacteria can acquire new genes by taking up DNA molecules (e. Kristin Hennessy-McDonald as she introduces our latest Learning Lab, the True Blue Transformation Lab: Genetically Engineering Bacteria. Transformation is the uptake of free Transformation of bacteria with plasmids is important not only for studies in bacteria but also because bacteria are used as the means for both storing and replicating plasmids. coli. This set of Microbiology Multiple Choice Questions & Answers (MCQs) focuses on “Bacterial Recombination”. Bacterial transformation, as mentioned above, means the uptake of DNA molecules through the cell wall from the external surroundings, followed by stable incorporation into the recipient genome, or replication as an independent plasmid. Bacteria बैक्टीरिया/ जीवाणुSexual reproduction (लैंगिक जनन)👉संयुग्मन (conjugation)👉रूपान्तरण Nov 24, 2019 · Bacteria are commonly used in the development of the DNA found inside cells. एन. It includesBacterial GeneticsTypes of Genetic Transfer in BacteriaBacterial Transfo Please click this hyperlink to access the video: bacterial transformation (plating cells) Clean your work surface, dispose of biological in the appropriate Biohazard disposal containers and wash your hands in the designated hand-washing sink. Bacterial Transformation. In nature, transformation can occur in certain types of bacteria. Recently, electroporation, or electr May 15, 2022 · Transformation. The top Bacterial transformation is a method of horizontal gene transfer whereby some bacteria take in unprocessed DNA from their environment. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research, scientific journals, and education. EN. Apr 16, 2020 · What is Bacterial Transformation? • Transformation is the direct uptake, incorporation and expression of exogenous genetic material from its surroundings. Transformation is the process where a bacterium takes up DNA from its environment. This process can re Jan 18, 2022 · रूपान्तरण (Transformation): कुछ जीवाणु अपने वातावरण में उपलब्ध DNA अणुओं के नग्न खण्डों को ग्रहण कर लेते हैं। ये DNA खण्ड जीवाणु के DNA अणु के समजात Figure 1: General steps of the transformation process. This video will explain these Get access to the latest Bacterial Transformation - 1 (in Hindi) prepared with CSIR-UGC NET course curated by Jyoti Kumari on Unacademy to prepare for the toughest competitive exam. S. What Makes a Bacteria Competent? Not all bacteria can naturally take up DNA from their environment. Some examples of pathogenic bacteria showing competence: Haemophilus spp; Streptococcus spp; Neisseria spp; Mechanism of Bacterial Transformation: In this particular example, we are going to observe how ampicillin-sensitive bacterial cells turn to ampicillin-resistant via uptake of ampicillin resistance gene through transformation. Let’s take a look at these different methods of DNA insertion. Hello everyone welcome back to my channel jiyashanky MEDICAL EDUCATION In this video I’ve been told about #bacterial#growth #bacterial#growth#in#hindi #bacte Nov 1, 2023 · Bacterial Transformation. in this video video we are d Join Dr. Dec 7, 2024 · Bacterial transformation is a critical process used to introduce foreign DNA into bacterial cells, enabling genetic manipulation for research and industrial applications. September 25, 2019 at 11:02 am. Different modes of horizontal g Lerminiaux, Nicole A. In this less May 18, 2023 · ग्राम स्टेनिंग सिद्धांत (Gram Staining Principle in hindi) (Gram Staining) ग्राम स्टेनिंग में बॅक्टेरिया के स्मियर को सबसे पहले प्रायमरी स्टेन क्रिस्टल वायलेट (Crystal Violet) से स्टेन किया May 22, 2024 · Griffith experiment | Bacterial transformation experiment - This lecture explains Griffith experiment | Bacterial transformation experiment. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. St bacterial transformation sentences in Hindi. Explained in easy language for beginners in Biotechnology and microbiology. 2019. Registration No 3,257,927) and Goldbio (U. all the methods including conjugation, transformation and transduction have been e MOLECULAR BIOLOGY by DL Kumhar (Biology Lecture) This is the video I have explained in Topic Evidences For DNA As Genetic Material (Experiments Of Griffith Get access to the latest Laboratory Protocol of Bacterial Transformation (In Hindi) prepared with CSIR-UGC NET course curated by Jyoti Kumari on Unacademy to prepare for the toughest competitive exam. com/topic. Record what did and did not grow on the agar plates, along with any relevant observations of the growth. During transformation, closely located genes are more likely to be taken up together, a process known as double transformation. com Feb 28, 2021 · Learning about Bacterial Genetic Recombination? This occurs by three mechanisms: Transduction, Transformation and Conjugation. Transformation, conjugation and transduction processes in bacteria occur in nature. 9 Why perform each transformation step? Incubation on ice slows fluid cell membranes Heat-shock increases the permeability of cell membrane to DNA. com Watch this JoVE video on Bacterial Transformation: The Heat Shock Method Watch Sexual Reproduction in Bacteria - Transformation and Transduction in Hindi from Microorganisms - Bacteria and Bacteria and Archaea and Transduction, Transformation and Conjugation here. Apr 7, 2021 · The plasmid often contains an antibiotic resistance gene. Bacterial transformation is a complex process encoded by numerous bacterial genes, and is a bacterial adaptation for DNA transfer. coli is the most common bacterial species used in the transformation step of a cloning workflow. In the lab, this process can be induced artificially, by using high voltage electric field pulses to create pores in the bacterial cell membrane, through which plasmid DNA can pass. https://dnalc. -Write Transfo Learn by watching this video about Bacterial Transformation using Plasmids - Prep at JoVE. • Transformation is a complex process. They have one circular chromosome and multiple small circular pieces of double-stranded DNA called plasmids within the cytoplasm. Often, we use plasmid Mar 28, 2024 · Bacterial transformation is a process wherein bacteria naturally modify their genetic makeup by incorporating foreign genetic material into their own genome. DNA analysis can confirm the gene’s presence in the plasmid. Increase the bacterial uptake of foreign DNA. Apr 12, 2017 · In this animation produced by WGBH and Digizyme, Inc. Bacteria take up the plasmid through transformation. Any genes (including antibiotic resistance genes) can be obtained by the uptake of free-floating DNA released from a bacterial cell that has died. Register for a free account at https://babec. . It starts with introduction. 8K Views. , and Andrew D. Competence is also discussed. They learn what role enzymes, DNA an Transformation in bacteria is a process by which bacteria takes up and incorporates foreign DNA from its environment into its own genome. Bacterial Reproduction or Horizontal Sep 18, 2020 · Bacterial Transformation, it's definition and mechanism. 點擊查看更多bacterial transformation Jul 17, 2015 · The electric pulses temporarily permeabilize the membrane, and the DNA enters through newly formed pores and incorporates into the host cell genome. Bacteria are the ideal organisms for transformation as they can easily take in exogenous genetic material into their genome and quickly amplify it 3,5. php?Title= Bacterial transformation is a form of horizontal gene transfer where competent cells uptake free or naked DNA from their environment. Recombinant DNA (rDNA): DNA made by connecting fragments from different sources. Transgenic organism: An organism with new genes which it expresses. com/channel/UC-VuLHRR-rfC2frh Apr 8, 2019 · Students construct paper recombinant plasmids to simulate the methods genetic engineers use to create modified bacteria. Videos Mapped to Your Course; Teacher Resources Get in Touch Instant Trial. In molecular biology, however, transformation is artificially induced through the creation of pores in the bacterial cell walls. com/playlist?list=PLdVf1SVYTjHMtB87K6csppEje59KP9n_6B. hfqdxj pbrqi ebhfda pwzfeuig wpdw hje etk qnrx jwy osdh